Aumento del daño en el ADN espermático en varones mayores de 40 años / Association of age with sperm DNA fragmentation
Rev. méd. Chile
;
139(3): 306-312, mar. 2011. ilus
Article
in Spanish
| LILACS
| ID: lil-597618
ABSTRACT
Background:
There is an association between aging ana an increased number ofsperms with alterations in nuclear DNA.Aim:
To study the association between age and fragmentation of sperm DNA. Material andMethods:
Sixty two volunteers provided semen for analysis. These were separated in a group aged less than forty years and a second group aged more than forty years. Sperm DNA fragmentation was studied by TÚNEL (terminal deoxynucleotidyl transferase-mediated2'-deoxyuridine 5'-triphosphate nick end-labeling) and SCD (sperm chromatin dispersión test) assays.Results:
Compared with theiryounger counterparts, patients aged more than 40years had a higher proportion ofsperms with DNA fragmentation by TÚNEL (20 ±1.3 and24 ± 1.9 percent respectively, p < 0.05) and SCD (22 ± 1.4 and26 ± 1.6 percent respectively, p < 0.05). The results ofboth assays had a correlation coefficientofO.8. No differences between groups were observed for other seminal parameters.Conclusions:
Sperm DNA fragmentation increases with age in males.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Spermatozoa
/
Chromatin
/
In Situ Nick-End Labeling
/
DNA Fragmentation
/
Semen Analysis
Type of study:
Risk factors
Limits:
Adolescent
/
Adult
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2011
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de Chile/CL
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