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Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals
Pires, Regina Helena; Santos, Jaime Maia dos; Zaia, José Eduardo; Martins, Carlos Henrique Gomes; Mendes-Giannini, Maria José Soares.
  • Pires, Regina Helena; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara. BR
  • Santos, Jaime Maia dos; Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Fitossanidade Vegetal. Jaboticabal. BR
  • Zaia, José Eduardo; Universidade de Franca. Laboratório de Pesquisa em Microbiologia Aplicada. Franca. BR
  • Martins, Carlos Henrique Gomes; Universidade de Franca. Laboratório de Pesquisa em Microbiologia Aplicada. Franca. BR
  • Mendes-Giannini, Maria José Soares; Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas. Araraquara. BR
Mem. Inst. Oswaldo Cruz ; 106(6): 646-654, Sept. 2011. ilus, graf, tab
Article in English | LILACS | ID: lil-602046
ABSTRACT
Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.
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Full text: Available Index: LILACS (Americas) Main subject: Water Microbiology / Candida / Fluconazole / Amphotericin B / Renal Dialysis / Biofilms / Antifungal Agents Type of study: Prognostic study Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade de Franca/BR

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Full text: Available Index: LILACS (Americas) Main subject: Water Microbiology / Candida / Fluconazole / Amphotericin B / Renal Dialysis / Biofilms / Antifungal Agents Type of study: Prognostic study Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Estadual Paulista/BR / Universidade de Franca/BR