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Validation of an immunochromatographic assay kit for the identification of the Mycobacterium tuberculosis complex
Toihir, Al-Habib Omar Said; Rasolofo, Voahangy; Andrianarisoa, Samuel Hermas; Ranjalahy, Gabriel Marie; Ramarokoto, Herimanana.
  • Toihir, Al-Habib Omar Said; Institut Pasteur. Unité des Mycobactéries. MG
  • Rasolofo, Voahangy; Institut Pasteur. Unité des Mycobactéries. MG
  • Andrianarisoa, Samuel Hermas; Organisation Mondiale de la Santé. MG
  • Ranjalahy, Gabriel Marie; Ministère de la Santé. MG
  • Ramarokoto, Herimanana; Institut Pasteur. Unité des Mycobactéries. MG
Mem. Inst. Oswaldo Cruz ; 106(6): 777-780, Sept. 2011. tab
Article in English | LILACS | ID: lil-602066
ABSTRACT
The performance of the immunochromatographic assay, SD BIOLINE TB Ag MPT64 RAPID®, was evaluated in Madagascar. Using mouse anti-MPT64 monoclonal antibodies for rapid discrimination between the Mycobacterium tuberculosis complex and nontuberculous mycobacteria, the kit was tested on mycobacteria and other pathogens using conventional methods as the gold standard. The results presented here indicate that this kit has excellent sensitivity (100 percent) and specificity (100 percent) compared to standard biochemical detection and can be easily used for the rapid identification of M. tuberculosis complex.
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Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis / Chromatography, Affinity / Bacterial Typing Techniques / Nontuberculous Mycobacteria / Mycobacterium tuberculosis Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Country/Region as subject: Africa Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article Affiliation country: Burkina Faso / France / Madagascar Institution/Affiliation country: Institut Pasteur/MG / Ministère de la Santé/MG / Organisation Mondiale de la Santé/MG

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Full text: Available Index: LILACS (Americas) Main subject: Tuberculosis / Chromatography, Affinity / Bacterial Typing Techniques / Nontuberculous Mycobacteria / Mycobacterium tuberculosis Type of study: Diagnostic study / Prognostic study Limits: Animals / Humans Country/Region as subject: Africa Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2011 Type: Article Affiliation country: Burkina Faso / France / Madagascar Institution/Affiliation country: Institut Pasteur/MG / Ministère de la Santé/MG / Organisation Mondiale de la Santé/MG