An enzyme-linked lectin assay for the study of lectin receptors of Leishmania
Braz. j. med. biol. res
;
21(3): 517-21, Mar. 1988. tab
Article
in English
| LILACS
| ID: lil-60240
RESUMO
An enzyme-linked lectin assay (ELLA) based on the ELISA assay, using intact formalin-fixed promastigotes to coat poly-L-lysine-treated microtiter plates is described. The assay was used to study the lectin receptors of Leishmania donovani chagasi, L. donovani donovani and L. mexicana amazonensis. ConA, RCA, WGA, and PNA receptors were found in the three parasites. SBA receptors were found to be as frequent as the other receptors in L. donovani chagasi but not in the other two parasites which showed little SBA binding. Trypsin treatment of the two L. donovani subspecies did not remove any of the lectin receptors studied
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Index:
LILACS (Americas)
Main subject:
Leishmania donovani
/
Leishmania mexicana
/
Enzyme-Linked Immunosorbent Assay
/
Receptors, Mitogen
Limits:
Animals
Language:
English
Journal:
Braz. j. med. biol. res
Journal subject:
Biology
/
Medicine
Year:
1988
Type:
Article
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