Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
Electron. j. biotechnol
; 14(3): 6-6, May 2011. ilus, tab
Article
in En
| LILACS
| ID: lil-602983
Responsible library:
CL1.1
ABSTRACT
The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95 percent purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for maximizing production and purification of proteins expressed as inclusion bodies in E. coli.
Key words
Full text:
1
Index:
LILACS
Main subject:
Muramidase
/
Inclusion Bodies
/
Spodoptera
Language:
En
Journal:
Electron. j. biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2011
Type:
Article