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A preferable approach to clone hLIF cDNA from the genomic DNA
Cui, Yihong; Zhu, Guangqin; Chen, Qiuju; Wang, Yunfei; Yang, Mingming; Song, Yuxuan; Wang, Jiangang; Cao, Binyun.
  • Cui, Yihong; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Zhu, Guangqin; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Chen, Qiuju; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Wang, Yunfei; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Yang, Mingming; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Song, Yuxuan; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Wang, Jiangang; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
  • Cao, Binyun; Northwest Agriculture and Forestry University. College of Animal Science and Technology. Yangling. CN
Electron. j. biotechnol ; 14(3): 12-12, May 2011. ilus, tab
Article in English | LILACS | ID: lil-602989
ABSTRACT
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.
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Full text: Available Index: LILACS (Americas) Main subject: DNA, Complementary / Leukemia Inhibitory Factor / Mouth Mucosa Limits: Humans Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2011 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Northwest Agriculture and Forestry University/CN

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Full text: Available Index: LILACS (Americas) Main subject: DNA, Complementary / Leukemia Inhibitory Factor / Mouth Mucosa Limits: Humans Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2011 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Northwest Agriculture and Forestry University/CN