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Effect of laser on the remnant liver after the first 24 hours following 70 percent hepatectomy in rats / Efeito do laser no fígado remanescente nas primeiras 24 horas após hepatectomia a 70 por cento em ratos
Barbosa, Ailton Jose; Santana, Ana Carolina Delazia Albuquerque; Castro e Silva, Tiago; Kurachi, Cristina; Inada, Natalia; Bagnato, Vanderlei Salvador; Silva Júnior, Orlando de Castro e.
  • Barbosa, Ailton Jose; USP. FMRP. Department of Surgery and Anatomy. Ribeirao Preto. BR
  • Santana, Ana Carolina Delazia Albuquerque; USP. FMRP. Ribeirao Preto. BR
  • Castro e Silva, Tiago; Illinois Institute of Technology. Department of Applied Mathematics. Chicago. US
  • Kurachi, Cristina; USP. Institute of Physics of Sao Carlos. Department of Biophotonics. Sao Carlos. BR
  • Inada, Natalia; USP. Institute of Physics of Sao Carlos. Department of Biophotonics. Sao Carlos. BR
  • Bagnato, Vanderlei Salvador; USP. Institute of Physics of Sao Carlos. Sao Carlos. BR
  • Silva Júnior, Orlando de Castro e; USP. FMRP. Chairman and Head, Department of Surgery and Anatomy. Ribeirao Preto. BR
Acta cir. bras ; 26(6): 470-474, Nov.-Dec. 2011. ilus
Article in English | LILACS | ID: lil-604196
ABSTRACT

PURPOSE:

To evaluate the mitochondrial function of the remnant liver (RL) in the early phase of liver regeneration in rats after 70 percent partial hepatectomy (PH).

METHODS:

Sixty male Wistar rats (200-250g) submitted to 70 percent PH were divided into five groups according to the time of euthanasia and application or not of laser light C = Control, time zero; 2 minutes, 4, 6 and 24 hours after PH. The dose of laser radiation was 22.5 J/cm², wavelength of 660 nm (visible/red), in the remnant liver. We studied the respiration activated by ADP (state 3), basal mitochondrial respiration (state 4), respiratory control ratio (RCR) and mitochondrial membrane potential (MMP).

RESULTS:

The mitochondrial function of RL changed at 4 and 6 hours after PH, with a significant increase in state 3 and a concomitant increase in state 4 and with maintenance of RCR. MMP differed significantly between the groups biostimulated with laser radiation and the control group 4 hours after HP, with a substantial reduction in the non-laser groups.

CONCLUSION:

The laser light at the dose used in this study did not induce additional damage to the RL and seems to have delayed the hepatocellular metabolic overload of the remnant liver.
RESUMO

OBJETIVO:

Avaliar a função mitocondrial do fígado remanescente (FR) na fase precoce da regeneração hepática em ratos após hepatectomia parcial (HP) a 70 por cento.

MÉTODOS:

Sessenta ratos machos Wistar (200 - 250g) submetidos à HP a 70 por cento, foram distribuídos em cinco grupos de acordo com o tempo de eutanásia e com aplicação ou não de luz laser C= Controle,tempo zero; 2 minutos, 4, 6 e 24 horas após HP. O laser foi utilizado na dose 22.5 J/cm², 660 nm, no FR.Estudou-se o estado 3 (respiração ativada por ADP), estado 4 (respiração mitocondrial basal), razão de controle respiratório,estado 3/estado 4 (RCR) e o potencial de membrana mitocondrial(PMM).

RESULTADOS:

A função mitocondrial do FR alterou-se no período de 4 e 6 horas após a HP com aumento significativo do estado 3 e aumento concomitante do estado 4, com manutenção da RCR. O PMM apresentou diferença significativa entre os grupos bioestimulados com laser e o controle a partir de 4 horas pós HP, com queda importante do grupo sem laser e tendência a equiparação dos valores após 24 horas.

CONCLUSÃO:

A luz laser, na dose utilizada no presente estudo, não induziu lesão adicional ao FR e parece ter retardado a sobrecarga hepatocelular do fígado remanescente.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Mitochondria, Liver / Membrane Potential, Mitochondrial / Laser Therapy / Hepatectomy / Liver Regeneration Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2011 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Illinois Institute of Technology/US / USP/BR

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Full text: Available Index: LILACS (Americas) Main subject: Mitochondria, Liver / Membrane Potential, Mitochondrial / Laser Therapy / Hepatectomy / Liver Regeneration Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2011 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Illinois Institute of Technology/US / USP/BR