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Isolation of endophytic bacteria from arboreal species of the Amazon and identification by sequencing of the 16S rRNA encoding gene
Coêlho, Mariza M; Ferreira-Nozawa, Monica S; Nozawa, Sérgio R; Santos, André L. W.
  • Coêlho, Mariza M; Universidade Nilton Lins. Laboratório de Biotecnologia Vegetal. Manaus. BR
  • Ferreira-Nozawa, Monica S; Universidade Nilton Lins. Laboratório de Expressão Gênica. Manaus. BR
  • Nozawa, Sérgio R; Universidade Nilton Lins. Laboratório de Expressão Gênica. Manaus. BR
  • Santos, André L. W; Universidade Nilton Lins. Laboratório de Biotecnologia Vegetal. Manaus. BR
Genet. mol. biol ; 34(4): 676-680, 2011. ilus, tab
Article in English | LILACS | ID: lil-605939
ABSTRACT
Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species.
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Full text: Available Index: LILACS (Americas) Main subject: Bacillus / Sequence Analysis, DNA / Pantoea Type of study: Diagnostic study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Nilton Lins/BR

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Full text: Available Index: LILACS (Americas) Main subject: Bacillus / Sequence Analysis, DNA / Pantoea Type of study: Diagnostic study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade Nilton Lins/BR