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The effects of electromagnetic fields on the ultrastructure of heart / Efectos de los campos electromagnéticos en la ultraestructura del corazón
Sõker, Sevda; Sert, Cemil; Deniz, Mustafa; Tekmen, Is¸il; Akkus, Murat; Nergiz, Yusuf.
  • Sõker, Sevda; Dicle University. Medicine Faculty. Department of Histology and Embryology. Diyarbakir. TR
  • Sert, Cemil; Harran University. Medicine Faculty. Department of Biophysics. Sanliurfa. TR
  • Deniz, Mustafa; Harran University. Medicine Faculty. Department of Anatomy. Sanliurfa. TR
  • Tekmen, Is¸il; Dokuz Eylül University. Medicine Faculty. Department of Histology and Embryology. Izmir. TR
  • Akkus, Murat; Dicle University. Medicine Faculty. Department of Histology and Embryology. Diyarbakir. TR
  • Nergiz, Yusuf; Dicle University. Medicine Faculty. Department of Histology and Embryology. Diyarbakir. TR
Int. j. morphol ; 29(3): 960-964, Sept. 2011. ilus
Article in English | LILACS | ID: lil-608689
ABSTRACT
Increasingly the use and convenience of electrical appliances in our daily lives are the cause of harmful effects caused by electromagnetic fields (EMF). The aim of this study was to research the effect of EMF on the ultrastructure of the heart in EMF exposed rats. In this study 45 male Sprague Dawley rats ranging in weight between 260 and 280 grams were used. The rats were divided into 3 groups, control (n15), Sham (n15) and EMF group (n 15) and exposed for 14 days 3 hours per day; gauss levels at 2.5 were applied to the EMF group, while the sham group in the same environment in Plexiglas cage was kept for 14 days 3 hours per day without magnetic field exposure. Control group at 14/10 hours light dark cycle fed in normal cages for 14 days. After two weeks rats were sacrificed by 50mg/kg of Ketalar anesthesia and heart tissue fixed in 2.5 gluteraldehide. Routine follow up with electron microscopic assessment. Mitochondrial structures and cellular structures observed in all the groups were normal. Myofibrillar loss, dilatation of smooth endoplasmic reticulum, mitochondrial swelling or cristalysis was not observed. Intercalated disc degeneration and apoptosis of nucleus was not observed. Therefore, and as a result of our study we did not observe differences between control and EMF groups.
RESUMEN
El uso y la comodidad de los aparatos eléctricos en nuestra vida cotidiana cada vez más son causa de efectos perjudiciales debido a los campos electromagnéticos(CEM).El objetivo de este estudio fue investigar el efecto de los CEM sobre la ultraestructura del corazón en ratas. Fueron utilizadas 45ratas Sprague Daw ley, con peso entre 260 y 280gramos. Las ratas fueron divididas en 3 grupos control (n 15); Sham (n15), y grupo expuesto a CEM (n15) durante 14 días,3 horas por día. Se aplicó niveles de 2,5gaussal grupo expuesto a CEM, mientras que el grupo de tratamiento simulado en el mismo entorno en jaulas plexiglás se mantuvo durante 14 días 3 horas día, sin exposición a campo electromagnético. Grupo control alimentado en jaulas normales durante 14 días con ciclo luz/oscuridad de 14/10. Al termino de dos semanas las ratas fueron sacrificadas por medio de anestesia Ketalar 50mg/kg y el tejido del corazón fijado engluteraldehido al 2,5. Se realizó seguimiento de rutina con correspondiente evaluación de microscopía electrónica. Las estructuras mitocondriales y celular es observadas en todos los grupos eran normales. No se observó pérdida miofibrilar, tampoco aumento del volumen mitocondrial ni dilatación del retículo endoplásmico lisoocristalysis. No se observó degeneración de los discosintercaladoso apoptosis de núcleo. Por lo tanto,y como resultado de nuestro estudio no encontramos diferencias entre los grupos control y CEM.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Myocytes, Cardiac / Electromagnetic Fields Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2011 Type: Article Affiliation country: Turkey Institution/Affiliation country: Dicle University/TR / Dokuz Eylül University/TR / Harran University/TR

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Full text: Available Index: LILACS (Americas) Main subject: Myocytes, Cardiac / Electromagnetic Fields Limits: Animals Language: English Journal: Int. j. morphol Journal subject: Anatomy Year: 2011 Type: Article Affiliation country: Turkey Institution/Affiliation country: Dicle University/TR / Dokuz Eylül University/TR / Harran University/TR