Trypanosoma cruzi trypomastigotes induce cytoskeleton modifications during HeLa cell invasion
Mem. Inst. Oswaldo Cruz
;
106(8): 1014-1016, Dec. 2011. ilus
Article
in English
| LILACS
| ID: lil-610979
ABSTRACT
It has been recently shown that Trypanosoma cruzi trypomastigotes subvert a constitutive membrane repair mechanism to invade HeLa cells. Using a membrane extraction protocol and high-resolution microscopy, the HeLa cytoskeleton and T. cruzi parasites were imaged during the invasion process after 15 min and 45 min. Parasites were initially found under cells and were later observed in the cytoplasm. At later stages, parasite-driven protrusions with parallel filaments were observed, with trypomastigotes at their tips. We conclude that T. cruzi trypomastigotes induce deformations of the cortical actin cytoskeleton shortly after invasion, leading to the formation of pseudopod-like structures.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Cytoskeleton
/
Cell Membrane
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2011
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Universidade Federal de São Paulo/BR
/
University of Maryland/US
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