Amylase production by solid-state fermentation of agro-industrial wastes using Bacillus sp
Braz. j. microbiol
;
42(4): 1334-1342, Oct.-Dec. 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-614593
ABSTRACT
Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 13 moisture content, 20 percent inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50ºC and 6 respectively. The enzyme was found to be thermostable at 70ºC for about 2 h without any salt. It showed stability at pH range 5-7. The metal ions as Na+, Ca++, Mg++ and Co++ enhanced the enzyme activity.
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Index:
LILACS (Americas)
Main subject:
Soil
/
Soil Conditions
/
Enzyme Activation
/
Agribusiness
/
Fermentation
/
Garbage
/
Amylases
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2011
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Amity University/IN
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