Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Braz. j. microbiol
;
42(4): 1440-1444, Oct.-Dec. 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-614608
ABSTRACT
Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Respirovirus Infections
/
Phylogeny
/
In Vitro Techniques
/
Base Sequence
/
Reverse Transcriptase Polymerase Chain Reaction
/
Reverse Transcription
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Sul/BR
/
Universidade Feevale/BR
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