Antibiotic resistance of Vibrio parahaemolyticus isolated from pond-reared Litopenaeus vannamei marketed in Natal, Brazil
Braz. j. microbiol
;
42(4): 1463-1469, Oct.-Dec. 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-614611
ABSTRACT
Ten out of fifty fresh and refrigerated samples of shrimp (Litopenaeus vannamei) collected from retailers in Natal (Rio Grande do Norte, Northeastern Brazil) tested positive for Vibrio parahaemolyticus. The Kanagawa test and multiplex PCR assays were used to detect TDH and TRH hemolysins and the tdh, trh and tlh genes, respectively. All strains were Kanagawa-negative and tlh-positive. Antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. Five strains (50 percent) presented multiple antibiotic resistance to ampicillin (90 percent) and amikacin (60 percent), while two strains (20 percent) displayed intermediate-level resistance to amikacin. All strains were sensitive to chloramphenicol. Intermediate-level susceptibility and/or resistance to other antibiotics ranged from 10 to 90 percent, with emphasis on the observed growing intermediate-level resistance to ciprofloxacin. Half our isolates yielded a multiple antibiotic resistance index above 0.2 (range 0.14-0.29), indicating a considerable risk of propagation of antibiotic resistance throughout the food chain.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Vibrio parahaemolyticus
/
In Vitro Techniques
/
Drug Resistance, Microbial
/
Polymerase Chain Reaction
/
Penaeidae
/
Disease Susceptibility
/
Hemolysin Proteins
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2011
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Rio Grande do Norte/BR
/
Universidade Potiguar/BR
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