Your browser doesn't support javascript.
loading
Gamma-tocotrienol modulation of senescence-associated gene expression prevents cellular aging in human diploid fibroblasts
Makpol, Suzana; Zainuddin, Azalina; Chua, Kien Hui; Yusof, Yasmin Anum Mohd; Ngah, Wan Zurinah Wan.
  • Makpol, Suzana; Universiti Kebangsaan Malaysia. Faculty of Medicine. Department of Biochemistry. Jalan Raja Muda Abdul Aziz. MY
  • Zainuddin, Azalina; Universiti Kebangsaan Malaysia. Faculty of Medicine. Department of Biochemistry. Jalan Raja Muda Abdul Aziz. MY
  • Chua, Kien Hui; Universiti Kebangsaan Malaysia. Faculty of Medicine. Department of Physiology. Jalan Raja Muda Abdul Aziz. MY
  • Yusof, Yasmin Anum Mohd; Universiti Kebangsaan Malaysia. Faculty of Medicine. Department of Biochemistry. Jalan Raja Muda Abdul Aziz. MY
  • Ngah, Wan Zurinah Wan; Universiti Kebangsaan Malaysia. Faculty of Medicine. Department of Biochemistry. Jalan Raja Muda Abdul Aziz. MY
Clinics ; 67(2): 135-143, 2012. ilus, graf, tab
Article in English | LILACS | ID: lil-614637
ABSTRACT

OBJECTIVE:

Human diploid fibroblasts undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular aging. The beneficial effects of vitamin E in aging have been established, but studies to determine the mechanisms of these effects are ongoing. This study determined the molecular mechanism of γ-tocotrienol, a vitamin E homolog, in the prevention of cellular aging in human diploid fibroblasts using the expression of senescence-associated genes.

METHODS:

Primary cultures of young, pre-senescent, and senescent fibroblast cells were incubated with γ-tocotrienol for 24 h. The expression levels of ELN, COL1A1, MMP1, CCND1, RB1, and IL6 genes were determined using the quantitative real-time polymerase chain reaction. Cell cycle profiles were determined using a FACSCalibur Flow Cytometer.

RESULTS:

The cell cycle was arrested in the G0/G1 phase, and the percentage of cells in S phase decreased with senescence. CCND1, RB1, MMP1, and IL6 were upregulated in senescent fibroblasts. A similar upregulation was not observed in young cells. Incubation with γ-tocotrienol decreased CCND1 and RB1 expression in senescent fibroblasts, decreased cell populations in the G0/G1 phase and increased cell populations in the G2/M phase. γ-Tocotrienol treatment also upregulated ELN and COL1A1 and downregulated MMP1 and IL6 expression in young and senescent fibroblasts.

CONCLUSION:

γ-Tocotrienol prevented cellular aging in human diploid fibroblasts, which was indicated by the modulation of the cell cycle profile and senescence-associated gene expression.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Vitamin E / Cell Cycle / Chromans / Cellular Senescence / Beta-Galactosidase / Fibroblasts / Antioxidants Type of study: Risk factors Limits: Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2012 Type: Article Affiliation country: Malaysia Institution/Affiliation country: Universiti Kebangsaan Malaysia/MY

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Vitamin E / Cell Cycle / Chromans / Cellular Senescence / Beta-Galactosidase / Fibroblasts / Antioxidants Type of study: Risk factors Limits: Humans Language: English Journal: Clinics Journal subject: Medicine Year: 2012 Type: Article Affiliation country: Malaysia Institution/Affiliation country: Universiti Kebangsaan Malaysia/MY