STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis
Genet. mol. biol
;
35(1): 18-26, 2012. tab
Article
in English
| LILACS
| ID: lil-616982
ABSTRACT
STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach was used to assess the protein expression profile associated with STGC3-mediated apoptosis. Western blotting confirmed the differential up-regulation of prohibitin seen in proteomic analysis. These results indicate that overexpression of STGC3 inhibits xenograft growth in nude mice by enhancing apoptotic cell death through altered expression of apoptosis-related proteins such as Bcl-2, Bax and prohibitin. These data contribute to our understanding of the function of STGC3 in human nasopharyngeal carcinoma and provide new clues for investigating other STGC3-associated tumors.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Nasopharyngeal Neoplasms
/
Genes, Tumor Suppressor
/
Neoplasm Proteins
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2012
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Central South University/CN
/
The First People's Hospital of Yunnan Province/CN
/
University of South China/CN
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