Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest
Genet. mol. biol
;
35(1): 65-73, 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-616983
ABSTRACT
The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12 percent of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there was evidence of a bottleneck (HYBRIDLAB, BOTTLENECK software) that might influence the future viability of the population in south Brazil. 106 alleles were identified, with the number of alleles/locus ranging from 2 to 11. Mean observed heterozygosity, mean number of alleles and polymorphism information content were 0.609, 5.89, and 0.6255, respectively. This population presented evidence of a recent bottleneck and loss of genetic variation. Persistent regional poaching constitutes an increasing in the extinction risk.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Variation
/
Microsatellite Repeats
/
Puma
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2012
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Serrano de Conservação da Natureza/BR
/
Projeto Puma/BR
/
Universidade Federal do Rio Grande do Sul/BR
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