Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1
Genet. mol. biol
;
35(1): 164-171, 2012. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-617000
ABSTRACT
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Prostatic Neoplasms
/
DNA Methylation
/
RNA Interference
Limits:
Humans
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2012
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Huazhong University of Science & Technology/CN
/
Xi'an Jiaotong University/CN
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