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Abnormalities of digestive tract innervation in rat fetus treated with ethylenethiourea / Anomalias da inervação do trato digestório de fetos de ratas expostas à etilenotioureia
Lemos, Sidney Pereira Pinto; Martins, José Luiz; Lemos, Patrícia Veruska Ribeiro Barbosa; Silva, Silvio Romero Gonçalves e; Santos, Fernando Leandro dos; Silva Júnior, Valdemiro Amaro da.
  • Lemos, Sidney Pereira Pinto; UNIVASF. BR
  • Martins, José Luiz; UNIFESP. Department of Surgery. Pediatric Surgery Division.
  • Lemos, Patrícia Veruska Ribeiro Barbosa; UNIVASF. BR
  • Silva, Silvio Romero Gonçalves e; UNIVASF. BR
  • Santos, Fernando Leandro dos; Federal Rural University. Department of Veterinary Medicine. Pernambuco. BR
  • Silva Júnior, Valdemiro Amaro da; Federal Rural University. Department of Animal Morphology and Physiology. BR
Acta cir. bras ; 27(3): 244-250, Mar. 2012. ilus, graf
Article in English | LILACS | ID: lil-617964
ABSTRACT

PURPOSE:

The pathophysiology of abnormalities associated with myenteric plexus lesions remains imperfectly understood. Such abnormalities have been correlated with subocclusive intestinal conditions in children with Hirschsprung's disease, cases of chronic constipation and, postoperatively, in cases of anorectal anomalies. This study evaluated abnormalities of the myenteric plexus in fetus from female rats that received ethylenethiourea.

METHODS:

Female rats were exposed to ethylenethiourea on the 11th day of pregnancy (experimental group) or to 0.9 percent physiological solution (control group). Abnormalities were only found in the experimental group. The digestive tract muscle layer was analyzed morphometrically and changes to the frequencies of nerve plexus cells and interstitial cells of Cajal were evaluated, using hematoxylin-eosin, S-100 protein, neuron-specific enolase and C-Kit, respectively.

RESULTS:

Muscle and skeletal abnormalities were observed in 100 percent, anorectal anomalies in 86 percent, absent tail in 71 percent, short tail in 29 percent, duodenal atresia in 5 percent, esophageal atresia in 5 percent and persistent omphalomesenteric duct in 5 percent. Histopathological analysis showed a thinner muscle layer associated with lower frequencies of ganglion cells and interstitial cells of Cajal, in all gastrointestinal tract.

CONCLUSION:

Severe nerve plexus abnormalities associated with muscle layer atrophy were observed throughout the gastrointestinal tract in newborn rats exposed to ethylenethiourea.
RESUMO

OBJETIVO:

As anomalias associadas a lesões dos plexos mioentéricos permanecem sem plena compreensão da sua fisiopatologia. Alterações nos plexos nervosos têm sido correlacionadas com quadros suboclusivos intestinais em crianças portadoras de doença de Hirschsprung, em constipação crônica e no pós-operatório de anomalias anorretais. Este estudo avaliou as anomalias do plexo mioentérico em fetos de ratos fêmea que ingeriram etilenotioureia (ETU).

MÉTODOS:

Ratos fêmea foram expostos no 11º dia de gestação a ETU 1 por cento no Grupo Experimento e a solução fisiológica 0,9 por cento no Grupo Controle. Foram observadas anomalias apenas no Grupo experimento, sendo realizada morfometria da camada muscular e avaliadas alterações da frequência celular nos gânglios do plexo mioentérico e nas células intersticiais de Cajal (CIC) utilizando hematoxilina-eosina, P S-100, Enolase Neurônio Específica e C-KIT.

RESULTADOS:

Foram observadas anomalias musculoesqueléticas (100 por cento), anorretais (86 por cento), ausência de cauda (71 por cento), cauda curta (29 por cento), atresia duodenal (5 por cento), atresia esofágica (5 por cento) e conduto onfalomesentérico persistente (5 por cento). A análise histopatológica mostrou adelgaçamento da camada muscular associada às alterações da frequência das células ganglionares e das CIC em todos os segmentos do trato gastrointestinal.

CONCLUSÃO:

Foram observadas alterações graves nos plexos nervosos associadas ao adelgaçamento da camada muscular de todo o trato gastrointestinal nos fetos expostos a ETU.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Prenatal Exposure Delayed Effects / Abnormalities, Drug-Induced / Pregnancy / Muscular Atrophy / Digestive System Abnormalities / Ethylenethiourea / Myenteric Plexus Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal Rural University/BR / UNIVASF/BR

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Full text: Available Index: LILACS (Americas) Main subject: Prenatal Exposure Delayed Effects / Abnormalities, Drug-Induced / Pregnancy / Muscular Atrophy / Digestive System Abnormalities / Ethylenethiourea / Myenteric Plexus Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Federal Rural University/BR / UNIVASF/BR