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Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice / Implante de células tronco do tecido adiposo humano numa preparação de ácido hialurônico em camundongos
Dietrich, Isa; Cochet, Olivia; Villageois, Phi; Rodrigues, Consuelo Junqueira.
  • Dietrich, Isa; Sao Paulo University. School of Medicine. Department of Surgery. Laboratory of Surgical Anatomy of Human Structural Topography.
  • Cochet, Olivia; University of Nice Sophia-Antipolis. Institute of Developmental Biology and Cancer. Centre National de la Recherche Scientifique.
  • Villageois, Phi; University of Nice Sophia-Antipolis. Institute of Developmental Biology and Cancer. Centre National de la Recherche Scientifique.
  • Rodrigues, Consuelo Junqueira; USP. School of Medicine. Institute of Orthopedics and Traumatology. Spinal Cord Injury Laboratory.
Acta cir. bras ; 27(4): 283-289, Apr. 2012. ilus
Article in English | LILACS | ID: lil-622351
ABSTRACT

PURPOSE:

To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice.

METHODS:

Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies.

RESULTS:

Controls Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin- eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed.

CONCLUSION:

The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.
RESUMO

OBJETIVO:

Avaliar o implante de células tronco do tecido adiposo humano (CTTAH) em gel de ácido hialurônico (AH), injetados no tecido subcutâneo de camundongos atímicos.

MÉTODOS:

Implantes controle - HA com meio de cultura foram injetados no tecido subcutâneo da região infraescapular esquerda de 12 camundongos atímicos. Implantes de CTTAH HA com CTTAH suspensas em meio de cultura foi injetado no subcutâneo da região contra lateral, dos mesmos animais. Com oito semanas, os animais foram sacrificados e os implantes recuperados foram processados para extração de DNA genômico, estudo histológico por coloração por hematoxilina eosina e imnuoflurescência utilizando anticorpos anti vimentina humana e anti fator de von Willebrand.

RESULTADOS:

Controles - implantes não visualizados no local da injeção. Uma substância amorfa foi observada nos cortes corados por hematoxilina eosina. Vimentina humana e fator anti von Willebrand não foram identificados. DNA humano não foi detectado. Implantes de CTTAH - Uma massa era visível no local da injeção. Células fusiformes foram observadas nos corte corados com hematoxilina eosina. Tanto vimentina humana quanto fator de von Willebrand foram identificados pela imunofluorescência. A presença de DNA humano foi confirmada.

CONCLUSÃO:

O implante de células tronco do tecido adiposo humano em veículo de ácido hialurônico gel assegurou a manutenção das células no local do implante.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Adipose Tissue / Adipocytes / Stem Cell Transplantation / Hyaluronic Acid Type of study: Prognostic study Limits: Animals / Female / Humans Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil / France

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Full text: Available Index: LILACS (Americas) Main subject: Adipose Tissue / Adipocytes / Stem Cell Transplantation / Hyaluronic Acid Type of study: Prognostic study Limits: Animals / Female / Humans Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil / France