In vitro study of the effect of a probiotic bacterium Lactobacillus rhamnosus against herpes simplex virus type 1
Braz. j. infect. dis
;
16(2): 129-135, May-Apr. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-622732
ABSTRACT
BACKGROUND:
Due to the emergence of drug resistance in herpes simplex virus type 1 (HSV-1), researchers are trying to find other methods for treating herpes simplex virus type 1 infections. Probiotic bacteria are effective in macrophage activation and may have antiviral activities.OBJECTIVE:
This study aimed at verifying the direct effect of Lactobacillus rhamnosus, a probiotic bacterium, in comparison with Escherichia coli, a non-probiotic one, on HSV-1 infection, and determining its effect on macrophage activation for in vitro elimination of HSV-1 infection.METHODS:
The above bacteria were introduced into HSV-1 infected Vero cells, and their effects were examined using both MTT and plaque assay. To determine macrophage activation against in vitro HSV-1 infection, J774 cells were exposed to these bacteria; then, macrophage viability was examined with the MTT method, and tumor necrosis factor alpha (TNF-α), interferon-gamma (IFN-γ), and nitric oxide (NO) assessments were performed using the ELISA method.RESULTS:
A significant increased viability of macrophages was observed (p < 0.05) in the presence of Lactobacillus rhamnosus before and after HSV-1 infection when compared with Escherichia coli as a non-probiotic bacterium. However, tumor necrosis factor α concentration produced by Escherichia coli-treated J774 cells was significantly higher than Lactobacillus rhamnosus-treated J774 cells (p < 0.05). interferon-gamma and NO production were not different in the groups treated with Escherichia coli or with Lactobacillus rhamnosus.CONCLUSION:
The results of this study indicate that Lactobacillus rhamnosus enhances macrophage viability for HSV-1 elimination and activation against HSV-1 more effectively, when compared with non-probiotic Escherichia coli. it also seems that receptor occupation of macrophage sites decreases HSV-1 infectivity by both of the studied bacteria.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Herpesvirus 1, Human
/
Probiotics
/
Escherichia coli
/
Lacticaseibacillus rhamnosus
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2012
Type:
Article
Affiliation country:
Iran
Institution/Affiliation country:
Shiraz University of Medical Sciences/IR
/
Tabriz University of Medical Sciences/IR
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