Optimization of nutrition factors on chitinase production from a newly isolated Chitiolyticbacter meiyuanensis SYBC-H1
Braz. j. microbiol
;
43(1): 177-186, Jan.-Mar. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-622802
ABSTRACT
The present study reports statistical medial optimization for chitinase production by a novel bacterial strain isolated from soil recently, which the name Chitinolyticbacter meiyuanensis SYBC-H1 is proposed. A sequential statistical methodology comprising of Plackett-Burman and response surface methodology (RSM) was applied to enhance the fermentative production of chitinase, in which inulin was firstly used as an effective carbon source. As a result, maximum chitinase activity of 5.17 U/mL was obtained in the optimized medium, which was 15.5-fold higher than that in the basal medium. The triplicate verification experiments were performed under the optimized nutrients levels which indicated that it well agreed with the predicted value.
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Index:
LILACS (Americas)
Main subject:
Carbon
/
Chitinases
/
Data Interpretation, Statistical
/
Fermentation
/
Inulin
Type of study:
Prognostic study
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
Affiliation country:
China
/
United States
Institution/Affiliation country:
Anhui Agricultural University/CN
/
Jiangnam University/CN
/
Jiangsu Hanbon Science and Technology/CN
/
University of Massachusetts Medical School/US
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