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Effect of Bothrops alternatus snake venom on macrophage phagocytosis and superoxide production: participation of protein kinase C
Setubal, S. S; Pontes, A. S; Furtado, J. L; Kayano, A. M; Stábeli, R. G; Zuliani, J. P.
  • Setubal, S. S; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Pontes, A. S; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Furtado, J. L; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Kayano, A. M; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Stábeli, R. G; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
  • Zuliani, J. P; Tropical Pathology Research Institute. Laboratory of Biochemistry and Biotechnology and Laboratory of Cell Culture and Monoclonal Antibodies. BR
J. venom. anim. toxins incl. trop. dis ; 17(4): 430-441, 2011. graf
Article in English | LILACS | ID: lil-623506
ABSTRACT
Envenomations caused by different species of Bothrops snakes result in severe local tissue damage, hemorrhage, pain, myonecrosis, and inflammation with a significant leukocyte accumulation at the bite site. However, the activation state of leukocytes is still unclear. According to clinical cases and experimental work, the local effects observed in envenenomation by Bothrops alternatus are mainly the appearance of edema, hemorrhage, and necrosis. In this study we investigated the ability of Bothrops alternatus crude venom to induce macrophage activation. At 6 to 100 »g/mL, BaV is not toxic to thioglycollate-elicited macrophages; at 3 and 6 »g/mL, it did not interfere in macrophage adhesion or detachment. Moreover, at concentrations of 1.5, 3, and 6 »g/mL the venom induced an increase in phagocytosis via complement receptor one hour after incubation. Pharmacological treatment of thioglycollate-elicited macrophages with staurosporine, a protein kinase (PKC) inhibitor, abolished phagocytosis, suggesting that PKC may be involved in the increase of serum-opsonized zymosan phagocytosis induced by BaV. Moreover, BaV also induced the production of anion superoxide (O2-) by thioglycollate-elicited macrophages. This BaV stimulated superoxide production was abolished after treating the cells with staurosporine, indicating that PKC is an important signaling pathway for the production of this radical. Based on these results, we suggest that phagocytosis and reactive oxygen species are involved in the pathogenesis of local tissue damage characteristic of Bothrops spp. envenomations.
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Full text: Available Index: LILACS (Americas) Main subject: Phagocytosis / Protein Kinases / Bothrops / Crotalid Venoms / Macrophages / Animals, Poisonous Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Tropical Pathology Research Institute/BR

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Full text: Available Index: LILACS (Americas) Main subject: Phagocytosis / Protein Kinases / Bothrops / Crotalid Venoms / Macrophages / Animals, Poisonous Limits: Animals Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2011 Type: Article Affiliation country: Brazil Institution/Affiliation country: Tropical Pathology Research Institute/BR