Effects of irradiation and tunicamycin on the surface glycoproteins Schistosoma mansoni
Mem. Inst. Oswaldo Cruz
;
84(supl.1): 199-208, 1989. graf, ilus
Article
in English
| LILACS
| ID: lil-623579
ABSTRACT
The cercarial glycocalyx and schistosomulum surface contains a number of glycoproteins which are expressed in very variable amounts within a parasite population. Tunicamycin inhibits glycoprotein synthesis of schistosomula if the parasites are incubated for 24hr with the drug (10µg ml[raised to the power of -1]). An unexpected increase in lectin binding to the parasite surface was observed but no other changes were detected. Schistosomula treated in this way did not develop in the host past the lung stage. Ultraviolet irradiation (400µW min cm[raised to the power of-2]) also inhibited glycoprotein synthesis. Synthesis of other proteins, and in particular heat shock proteins, were also inhibited. Sera from mice (NIH strain) infected with irradiated cercariae contained antibodies which bound to normal schistosomula with lower affinity than to irradiated parasites. This is evidence that irradiation modifies the surface and secreted glycoproteins of schistosomula, so they are processed in a different way to normal glycoproteins by the host's immune system. The effects of irradiation on heat shock protein synthesis may allow the parasite to release a variety of proteins and glycoproteins in abnormal conformations. This may explain the enhanced immunogenicity of irradiated cercariae.
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Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Tunicamycin
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1989
Type:
Article
/
Congress and conference
Affiliation country:
Brazil
/
United kingdom
Institution/Affiliation country:
Universidade Federal de Minas Gerais/BR
/
University of Galsgow/GB
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