GP38, P28-I and P28-II: candidates for a vaccine against Schistosomiasis
Mem. Inst. Oswaldo Cruz
;
82(supl.4): 111-114, 1987.
Article
in English
| LILACS
| ID: lil-623673
ABSTRACT
Three antigens protective against Schistosoma mansoni have been extensively characterized. The schistosomulum surface antigen GP38 possesses an immunodominant carbohydrate epitope of which the structure has been defined. Protection can be achieved via the transfer of monoclonal antibodies recognizing the epitope or by immunization with anti-idiotype monoclonal antibodies. The glycan epitope is shared with the intermediate host, Biomphalaria glabrata as well as being present on other molluscs, including the Keyhole Limpet. A group of molecules at 28 kDa were initially characterized in adult worms and shown to protect rats and mice against a challenge infection. One of these molecules, P28-I, was cloned and expressed in E. coli, yeast and vaccinia virus. The recombinant antigen significantly protected rats, hamsters and baboons against a challenge infection. P28-I is a glutathione-S-transferase and the recombinant antigen produced in yeast exhibits the enzyme activity and has been purified to homogeneity by affinity chromatography. A second P28 antigen, P28-II, has also been cloned, fully sequenced and expressed. This recombinant antigen also protects against S. mansoni infection.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosomiasis mansoni
/
Antigens, Helminth
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1987
Type:
Article
/
Congress and conference
/
Project document
Affiliation country:
France
Institution/Affiliation country:
Institut Pasteur/FR
/
Transgène S A/FR
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