The use of monoclonal antibodies for the characterization and production of Mycobacterium leprae antigens
Mem. Inst. Oswaldo Cruz
;
82(supl.2): 147-151, 1987. tab
Article
in English
| LILACS
| ID: lil-623776
ABSTRACT
Similar immunizations of mice and hybridoma technology were used by several investigators to raise monoclonal antibodies which identified a limited range of epitopes and antigenic molecules. Further studies would have the scope for revealing yet more novel structures. The existing MABs are agreed standard reagents, avaiable to investigators and valuable for several applications. At least six epitopes specific for M. leprae were defined in molecular terms. Monoclonal antibody based immunoassays proved to be invaluable for the screening of recombinant DNA clones and for the topographic study of individual epitopes. Purification of antigens using affinity chromatography requires further development of techniques whilst serology of leprosy is open for clinical and epidemiological evaluation.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Antibodies, Monoclonal
/
Mycobacterium leprae
/
Antigens
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
1987
Type:
Article
/
Congress and conference
Affiliation country:
United kingdom
Institution/Affiliation country:
Hammersmith Hospital/GB
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