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Footprints of a trypanosomatid RNA world: pre-small subunit rRNA processing by spliced leader addition trans-splicing
Mayer, Mario Gustavo; Santos, Marcos Gonzaga dos; Silva, Maria Fernanda Laranjeira da; Floeter-Winter, Lucile Maria.
  • Mayer, Mario Gustavo; Instituto Butantan. Laboratório de Genética. São Paulo. BR
  • Santos, Marcos Gonzaga dos; Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo. BR
  • Silva, Maria Fernanda Laranjeira da; Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo. BR
  • Floeter-Winter, Lucile Maria; Universidade de São Paulo. Instituto de Biociências. Departamento de Fisiologia. São Paulo. BR
Mem. Inst. Oswaldo Cruz ; 107(4): 522-531, June 2012. ilus
Article in English | LILACS | ID: lil-626447
ABSTRACT
The addition of a capped mini-exon [spliced leader (SL)] through trans-splicing is essential for the maturation of RNA polymerase (pol) II-transcribed polycistronic pre-mRNAs in all members of the Trypanosomatidae family. This process is an inter-molecular splicing reaction that follows the same basic rules of cis-splicing reactions. In this study, we demonstrated that mini-exons were added to precursor ribosomal RNA (pre-rRNA) are transcribed by RNA pol I, including the 5' external transcribed spacer (ETS) region. Additionally, we detected the SL-5'ETS molecule using three distinct methods and located the acceptor site between two known 5'ETS rRNA processing sites (A' and A1) in four different trypanosomatids. Moreover, we detected a polyadenylated 5'ETS upstream of the trans-splicing acceptor site, which also occurs in pre-mRNA trans-splicing. After treatment with an indirect trans-splicing inhibitor (sinefungin), we observed SL-5'ETS decay. However, treatment with 5-fluorouracil (a precursor of RNA synthesis that inhibits the degradation of pre-rRNA) led to the accumulation of SL-5'ETS, suggesting that the molecule may play a role in rRNA degradation. The detection of trans-splicing in these molecules may indicate broad RNA-joining properties, regardless of the polymerase used for transcription.
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Full text: Available Index: LILACS (Americas) Main subject: Leishmania mexicana / RNA Precursors / RNA, Spliced Leader / Trans-Splicing Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Butantan/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Leishmania mexicana / RNA Precursors / RNA, Spliced Leader / Trans-Splicing Language: English Journal: Mem. Inst. Oswaldo Cruz Journal subject: Tropical Medicine / Parasitology Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Instituto Butantan/BR / Universidade de São Paulo/BR