Mycoplasma hyorhinis and Mycoplasma fermentans induce cell apoptosis and changes in gene expression profiles of 32D cells
Biol. Res
;
44(4): 383-391, 2011. ilus, tab
Article
in English
| LILACS
| ID: lil-626739
ABSTRACT
Infection of mycoplasmas has been linked to various human diseases including arthritis, pneumonia, infertility and cancer. While Mycoplasma hyorhinis and Mycoplasma fermentans have been detected in gastric adenocarcinomas, the mechanisms underlyine the pathogenesis are unknown. In this study, cell growth kinetics, Hoechst 33258 staining, DNA ladder assays, Western blotting analysis and cDNA microarray assays were performed to investigate the roles of M. hyorhinis and M. fermentans during infection of mammalian cells. Our data demonstrated that these mycoplasmas inhibid the growth of immortalised cell lines (32D and COS-7) ane tumor cell lines (HeLa and AGS). In addition, the infection of the 32D cell line with M. hyorhinis and M. fermentans induced compression of the nucleus, degradation of the cell genome and dysregulation of the expression of genes related to proliferation, apoptosis, tumorigenesis, signaling pathway and metabolism. Apoptosis related proteins Bcl-2, Bid and p53 were down-regulated, Fas was up-regulated and Bax was dysregulated in mycoplasma-infected 32D cells. Together, our data demonstrated that infection of mycoplasmas inhibitd cele growts through modification of gene expression profiles and post-translation modification of proliferation and apoptosis related proteins.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Mycoplasma fermentans
/
Apoptosis
/
Gene Expression Profiling
/
Mycoplasma hyorhinis
Limits:
Animals
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2011
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Peking University Cancer Hospital & Institute/CN
/
Wuhan Polytechnic University/CN
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