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Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay
Ellahueñe, Manuel F; Pérez-Alzola, L Patricia; Olmedo, M Isabel.
  • Ellahueñe, Manuel F; Universidad de Chile. Centro Nacional del Medio Ambiente. Unidad de Biodiversidad Acuática. Santiago. CL
  • Pérez-Alzola, L Patricia; Universidad Andrés Bello. Facultad de Ciencias Biológicas. Santiago. CL
  • Olmedo, M Isabel; Universidad de Chile. Centro Nacional del Medio Ambiente. Unidad de Biodiversidad Acuática. Santiago. CL
Biol. Res ; 45(1): 27-31, 2012. tab
Article in English | LILACS | ID: lil-626744
ABSTRACT
Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but observed MN frequencies were significantly lower than in the 38-week-old control group. This suggests a protective effect against genotoxic damage caused by aging, probably due to ethanol action as a hydroxyl radical scavenger. In the DLM assay, male mice drank ethanol at 15% or 30% (v/v) for 20 weeks. In both groups the number of dead implants was similar to the control, but there was a significant reduction in total implants, indicating a pre-implantation loss.
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Full text: Available Index: LILACS (Americas) Main subject: Bone Marrow / DNA Damage / DNA / Ethanol / Alcoholism / Mutation Type of study: Practice guideline / Prognostic study Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2012 Type: Article Affiliation country: Chile Institution/Affiliation country: Universidad Andrés Bello/CL / Universidad de Chile/CL

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Full text: Available Index: LILACS (Americas) Main subject: Bone Marrow / DNA Damage / DNA / Ethanol / Alcoholism / Mutation Type of study: Practice guideline / Prognostic study Limits: Animals Language: English Journal: Biol. Res Journal subject: Biology Year: 2012 Type: Article Affiliation country: Chile Institution/Affiliation country: Universidad Andrés Bello/CL / Universidad de Chile/CL