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Salmonella enterica serovar typhi plasmid pR ST98 enhances intracellular bacterial growth and S. typhi-induced macrophage cell death by suppressing autophagy
He, Peiyan; Wu, Shuyan; Chu, Yuanyuan; Yang, Yanru; Li, Yuanyuan; Huang, Rui.
  • He, Peiyan; Soochow University. Medical College. Microbiology Department. Suzhou. CN
  • Wu, Shuyan; Soochow University. Medical College. Microbiology Department. Suzhou. CN
  • Chu, Yuanyuan; Soochow University. Medical College. Microbiology Department. Suzhou. CN
  • Yang, Yanru; Soochow University. Medical College. Microbiology Department. Suzhou. CN
  • Li, Yuanyuan; Soochow University. Medical College. Microbiology Department. Suzhou. CN
  • Huang, Rui; Soochow University. Medical College. Microbiology Department. Suzhou. CN
Braz. j. infect. dis ; 16(3): 262-266, May-June 2012. ilus
Article in English | LILACS | ID: lil-638560
ABSTRACT

OBJECTIVES:

Plasmid pR ST98 is a hybrid resistance-virulence plasmid isolated from Salmonella enterica serovar Typhi (S. typhi). Previous studies demonstrated that pR ST98 could enhance the virulence of its host bacteria. However, the mechanism of pR ST98-increased bacterial virulence is still not fully elucidated. This study was designed to gain further insight into the roles of pR ST98 in host responses.

METHODS:

Human-derived macrophage-like cell line THP-1 was infected with wild-type (ST8), pR ST98-deletion (ST8-ΔpR ST98), and complemented (ST8-c-pR ST98) S. typhi strains. Macrophage autophagy was performed by extracting the membrane-unbound LC3-I protein from cells, followed by flow cytometric detection of the membrane-associated fraction of LC3-II. Intracellular bacterial growth was determined by colony-forming units (cfu) assay. Macrophage cell death was measured by flow cytometry after propidium iodide (PI) staining. Autophagy activator rapamycin (RAPA) was added to the medium 2 h before infection to investigate the effect of autophagy on intracellular bacterial growth and macrophage cell death after S. typhi infection.

RESULTS:

Plasmid pR ST98 suppressed autophagy in infected macrophages and enhanced intracellular bacterial growth and S. typhi-induced macrophage cell death. Pretreatment with RAPA effectively restricted intracellular bacterial growth of ST8 and ST8-c-pR ST98, and alleviated ST8 and ST8-c-pR ST98-induced macrophage cell death, but had no significant effect on ST8-ΔpR ST98.

CONCLUSIONS:

Plasmid pR ST98 enhances intracellular bacterial growth and S. typhi-induced macrophage cell death by suppressing autophagy.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Plasmids / Salmonella typhi / Autophagy / Bacterial Proteins / Apoptosis / Macrophages Limits: Humans Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2012 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Soochow University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Plasmids / Salmonella typhi / Autophagy / Bacterial Proteins / Apoptosis / Macrophages Limits: Humans Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2012 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Soochow University/CN