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Comparison of inflammatory microRNA expression in healthy and periodontitis tissues
Wa Lee, Young H; Am Na, Hee S; Jeong, So Y Eon; Jeong, Sung H Ee; Park, Hae R Youn; Chung, Jin.
  • Wa Lee, Young H; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Am Na, Hee S; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Jeong, So Y Eon; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Jeong, Sung H Ee; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Park, Hae R Youn; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Chung, Jin; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
Biocell ; 35(2): 43-49, Aug. 2011. graf, tab
Article in English | LILACS | ID: lil-639624
ABSTRACT
MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Thus, this study aimed to compare the miRNAs differentially expressed in healthy and chronic periodontitis tissues and to determine the miRNAs closely associated with chronic periodontitis. To find out the miRNAs differentially induced in healthy and chronic periodontitis tissues, miRNA microarray was carried out and the expression of miRNAs was confirmed by real-time PCR. According to miRNA microarray analyses, six miRNA genes, let-7a, let-7c, miR-130a, miR301a, miR-520d, and miR-548a, were up-regulated more than 8 fold compared to the healthy gingiva. The expression of twenty-two miRNAs was increased more than 4 fold. Among these miRNAs, eight miRNAs which are known to be closely related to inflammation were selected. Six of these miRNA genes, miR-181b, miR-19b, miR-23a, miR-30a, miR-let7a, and miR-301a, were amplified successfully and increased much more in periodontitis gingivae than in healthy ones. In summary, this study indicate that six miRNAs up-regulated in periodontitis gingiva may play a key role in chronic periodontitis.
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Full text: Available Index: LILACS (Americas) Main subject: Biomarkers / Gene Expression Profiling / MicroRNAs / Chronic Periodontitis / Gingiva / Inflammation Limits: Humans Language: English Journal: Biocell Journal subject: C‚lulas Year: 2011 Type: Article / Project document Affiliation country: South Korea Institution/Affiliation country: Pusan National University/KR

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Full text: Available Index: LILACS (Americas) Main subject: Biomarkers / Gene Expression Profiling / MicroRNAs / Chronic Periodontitis / Gingiva / Inflammation Limits: Humans Language: English Journal: Biocell Journal subject: C‚lulas Year: 2011 Type: Article / Project document Affiliation country: South Korea Institution/Affiliation country: Pusan National University/KR