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A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine / Avaliação dos níveis séricos de interleucina 6 (IL-6) e fator de necrose tumoral (TNF-α) em ratos submetidos a peritonite fecal e tratado com ropivacaína intraperitoneal
Brocco, Marcos Célio; Paulo, Danilo Nagib Salomão; Almeida, Carlos Eduardo David de; Carraretto, Antonio Roberto; Cabral, Sigmar Aurea; Silveira, Amanda Cardoso de Oliveira; Gomez, Renato Santiago; Baptista, João Florêncio de Abreu.
  • Brocco, Marcos Célio; Espirito Santo Federal University. Department of Surgery. BR
  • Paulo, Danilo Nagib Salomão; EMESCAM. Espirito Santo. BR
  • Almeida, Carlos Eduardo David de; UFMG. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Minas Gerais. BR
  • Carraretto, Antonio Roberto; Espirito Santo Federal University. Department of Surgery. BR
  • Cabral, Sigmar Aurea; UFMG. Surgical and Ophthalmological Applied Sciences Postgraduate Program. Minas Gerais. BR
  • Silveira, Amanda Cardoso de Oliveira; s.af
  • Gomez, Renato Santiago; Minas Gerais Federal University. Department of Surgery. BR
  • Baptista, João Florêncio de Abreu; Espirito Santo Federal University. Department of Surgery. BR
Acta cir. bras ; 27(7): 494-498, jul. 2012. tab
Article in English | LILACS | ID: lil-640099
ABSTRACT

PURPOSE:

The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage.

METHODS:

We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups I-control, no treatment; II- drying of the abdominal cavity; III- lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV- lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized.

RESULTS:

The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6, the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV.

CONCLUSION:

Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats.
RESUMO

OBJETIVO:

O objetivo do presente estudo foi avaliar as dosagens séricas das citocinas Il-6 e TNF-α em ratos submetidos à peritonite fecal e tratados com lavagem peritoneal com ropivacaína a 0,2%.

MÉTODOS:

Utilizaram-se 16 ratos Wistar, submetidos à laparotomia 6 horas após a indução de peritonite fecal com fezes autógenas, distribuídos aleatoriamente em 4 grupos I- Controle, nenhum tratamento; II- Enxugamento da cavidade abdominal; III- Lavagem da cavidade abdominal com 3 ml de solução salina 0,9% e enxugamento; IV- Lavagem da cavidade abdominal com 3 ml de ropivacaína a 0,2% e enxugamento. Seis horas após a laparotomia os animais foram submetidos à punção cardíaca com retirada de 1 mL de sangue para a dosagem das citocinas e, a seguir, eutanasiados.

RESULTADOS:

A lavagem com ropivacaína apresentou valores de TNF-α menores do que os observados com os outros tratamentos (p<0,05). Em relação aos valores da IL-6, o grupo da ropivacaína apresentou valores menores do que os observados com os grupos I e II, mas não houve diferença estatística (p>0,05) em relação ao grupo III.

CONCLUSÃO:

A lavagem peritoneal com ropivacaína a 0,2% no tratamento da peritonite fecal em ratos demonstrou reduzir os níveis plasmáticos de IL-6 e do TNF-α.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Peritonitis / Peritoneal Lavage / Tumor Necrosis Factor-alpha / Amides / Anesthetics, Local Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: EMESCAM/BR / Espirito Santo Federal University/BR / Minas Gerais Federal University/BR / UFMG/BR

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Full text: Available Index: LILACS (Americas) Main subject: Peritonitis / Peritoneal Lavage / Tumor Necrosis Factor-alpha / Amides / Anesthetics, Local Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: EMESCAM/BR / Espirito Santo Federal University/BR / Minas Gerais Federal University/BR / UFMG/BR