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Isolation of simple sequence repeats from groundnut
Tang Wang, Chuan; Dao Yang, Xin; Xu Chen, Dian; Lin Yu, Shan; Zhen Liu, Guang; Yi Tang, Yue; Zhi Xu, Jian.
  • Tang Wang, Chuan; Shandong Peanut Research Institute. China. CN
  • Dao Yang, Xin; Shandong Peanut Research Institute. China. CN
  • Xu Chen, Dian; Shandong Peanut Research Institute. China. CN
  • Lin Yu, Shan; Shandong Peanut Research Institute. China. CN
  • Zhen Liu, Guang; Shandong Peanut Research Institute. China. CN
  • Yi Tang, Yue; Shandong Peanut Research Institute. China. CN
  • Zhi Xu, Jian; Shandong Peanut Research Institute. China. CN
Electron. j. biotechnol ; 10(3): 473-480, July 2007. ilus, graf, tab
Article in English | LILACS | ID: lil-640488
ABSTRACT
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.


Full text: Available Index: LILACS (Americas) Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Shandong Peanut Research Institute/CN

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Full text: Available Index: LILACS (Americas) Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2007 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Shandong Peanut Research Institute/CN