Isolation of simple sequence repeats from groundnut
Electron. j. biotechnol
;
10(3): 473-480, July 2007. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-640488
ABSTRACT
SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2007
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Shandong Peanut Research Institute/CN
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