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Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L. ) inbred lines
González, Germán Andrés; Pacheco, María Gabriela; Oneto, Cecilia Décima; Etchart, Valeria J; Kandus, Mariana V; Salerno, Juan Carlos; Eyherabide, Guillermo; Presello, Daniel; Lewi, Dalia Marcela.
  • González, Germán Andrés; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Pacheco, María Gabriela; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Oneto, Cecilia Décima; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Etchart, Valeria J; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Kandus, Mariana V; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Salerno, Juan Carlos; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
  • Eyherabide, Guillermo; INTA. Estación Experimental Agropecuaria Pergamino. Buenos Aires. AR
  • Presello, Daniel; INTA. Estación Experimental Agropecuaria Pergamino. Buenos Aires. AR
  • Lewi, Dalia Marcela; INTA. Centro de Investigaciones en Ciencias Veterinarias y Agronómicas. Instituto de Genética Ewald A. Favret. Buenos Aires. AR
Electron. j. biotechnol ; 15(1): 9-9, Jan. 2012. ilus, tab
Article in English | LILACS | ID: lil-640535
ABSTRACT
Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC (mature somatic embryos/callus evaluated) x 100], Regeneration Capacity (RC) and Fertile Plant Recovery in greenhouse (FPR, fertile plants/regenerated plants) were recorded. A total of 17 lines reached an EC > 50 percent, and 14 out of those 17 lines regenerated seedlings. The FPR ranged between 50 and 100 percent. Also, we selected three promising lines with high agronomic performance, as alternatives to Hi II, in order to be included in a maize transformation scheme via somatic embryogenesis. In addition, we report the usefulness of Single Sequences Repeat (SSRs) in the determination of genetic diversity among 14 divergent lines for somatic embryogenesis response. The seven lines displaying good in vitro behaviour can be crossed to obtain hybrids combining desirable alleles for somatic embryogenesis response and different genetic backgrounds.
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Full text: Available Index: LILACS (Americas) Main subject: Regeneration / Zea mays / Plant Somatic Embryogenesis Techniques Type of study: Prognostic study Country/Region as subject: South America / Argentina Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2012 Type: Article / Project document Affiliation country: Argentina Institution/Affiliation country: INTA/AR

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Full text: Available Index: LILACS (Americas) Main subject: Regeneration / Zea mays / Plant Somatic Embryogenesis Techniques Type of study: Prognostic study Country/Region as subject: South America / Argentina Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2012 Type: Article / Project document Affiliation country: Argentina Institution/Affiliation country: INTA/AR