Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: the experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH
Genet. mol. res. (Online)
;
7(2): 417-423, 2008. tab
Article
in English
| LILACS
| ID: lil-640997
ABSTRACT
Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT until complete cytogenetic remission (CCR). We investigated the disease-free survival, overall survival in 3 years and transplant-related mortality rates, too. Bone marrow samples were collected at 1, 2, 3, 4, 6, 12, and 24 months after transplantation and additional intervals as necessary. Success rate of the FISH analyses was 100%. CCR was achieved in 75% of the patients, within on average of 3.9 months; 45% patients showed CCR within 60 days after HSCT. After 3 years of the allo-HSCT, overall survival rate was 60%, disease-free survival was 50% and the transplant-related mortality rate was 40%. The study demonstrated that the BCR-ABL FISH assay is useful for follow-up of chronic myeloid leukemia patients after HSCT and that the clinical outcome parameters in our patient cohort were similar to those described for other bone marrow transplantation units.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
/
In Situ Hybridization, Fluorescence
/
Hematopoietic Stem Cell Transplantation
Type of study:
Prognostic study
Limits:
Adolescent
/
Adult
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2008
Type:
Article
/
Project document
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Faculdade de Medicina de São José do Rio Preto/BR
/
Fundação Faculdade Regional de Medicina de São José do Rio Preto/BR
/
Universidade Estadual Paulista/BR
/
University of Colorado Comprehensive Cancer Center/US
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