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Chitosan: effect of a new chelating agent on the microhardness of root dentin
Pimenta, Josilaine Amaral; Zaparolli, Danilo; Pécora, Jesus Djalma; Cruz-Filho, Antonio Miranda.
  • Pimenta, Josilaine Amaral; University of São Paulo. Ribeirão Preto Dental School. Department of Restorative Dentistry. Ribeirão Preto. BR
  • Zaparolli, Danilo; University of São Paulo. Ribeirão Preto Dental School. Department of Restorative Dentistry. Ribeirão Preto. BR
  • Pécora, Jesus Djalma; University of São Paulo. Ribeirão Preto Dental School. Department of Restorative Dentistry. Ribeirão Preto. BR
  • Cruz-Filho, Antonio Miranda; University of São Paulo. Ribeirão Preto Dental School. Department of Restorative Dentistry. Ribeirão Preto. BR
Braz. dent. j ; 23(3): 212-217, 2012. ilus
Article in English | LILACS | ID: lil-641589
ABSTRACT
The effect of solutions of 0.2% chitosan, 15% EDTA and 10% citric acid on the microhardness of root dentin was evaluated comparatively in this study. Thirteen sound human maxillary central incisors were selected and decoronated at the cementoenamel junction. Ten roots were set into rapid polymerization acrylic resin and the root/resin block was fitted to the cutting machine to obtain slices from the cervical third. The first slice was discarded and the second slice was divided into four quadrants. Each quadrant was used to construct a sample, so that 4 specimens were obtained from each root slice, being one for each chelating solution to be tested 15% EDTA, 10% citric acid, 0.2% chitosan and distilled water (control). The specimens were exposed to 50 μL of the solution for 5 min, and then washed in distilled water. A microhardness tester (Knoop hardness) with a 10 g load was used for 15 s. Data were analyzed statistically by one-way ANOVA and Tukey-Kramer test (α=0.05). The other 3 roots had the canals instrumented and irrigated at the end of the biomechanical preparation with the test solutions, and then examined by scanning electron microscopy (SEM) for qualitative analysis. All solutions reduced the microhardness of root dentin in a way that was statistically similar to each other (p>0.05) but significantly different from the control (p>0.05). The SEM micrographs showed that the three solutions removed smear layer from the middle third of the root canal. In conclusion, 0.2% chitosan, 15% EDTA and 10% citric acid showed similar effects in reducing dentin microhardness.
RESUMO
Avaliou-se o efeito das soluções de quitosana 0,2%, EDTA 15% e ácido cítrico 10% sobre a microdureza da dentina radicular. Foram utilizados 13 incisivos centrais superiores humanos, os quais tiveram suas coroas seccionadas transversalmente e desprezadas. Dez raízes foram incluídas em resina acrílica de rápida polimerização e o bloco formado raiz/resina adaptado à maquina de corte. Desprezou-se o primeiro corte transversal da porção cervical e dividiu-se o segundo, em 4 quadrantes. Cada quarto foi destinado à confecção do corpo-de-prova obtendo-se 4 espécimes para cada raiz, um para cada solução (n=10) EDTA a 15%, ácido cítrico a 10%, quitosana a 0,2% e água destilada (controle). Os espécimes receberam 50 μ L da solução por 5 min, sendo em seguida, lavados com água destilada. Utilizou-se um microdurômetro (dureza Knoop) com carga de 10 g durante 15 s. Os dados foram avaliados por meio do teste ANOVA e Tukey-Kramer (α=0,05). Três incisivos centrais superiores foram instrumentados e irrigados, ao final da biomecânica, com uma das soluções estudadas. Os espécimes foram levados para MEV e posterior análise qualitativa. Todas as soluções avaliadas reduziram a microdureza da dentina radicular de forma semelhante entre si (p>0,05) e estatisticamente diferente do controle (p<0,01). As fotomicrografias mostraram que as 3 soluções removeram a smear layer do terço médio do canal radicular. Concluiu-se que as soluções de quitosana 0,2%, EDTA 15% e ácido cítrico 10% apresentam efeito semelhante na redução da microdureza dentinária.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Root Canal Irrigants / Tooth Root / Chelating Agents / Edetic Acid / Citric Acid / Dentin / Chitosan Type of study: Observational study / Prevalence study / Qualitative research Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Root Canal Irrigants / Tooth Root / Chelating Agents / Edetic Acid / Citric Acid / Dentin / Chitosan Type of study: Observational study / Prevalence study / Qualitative research Limits: Humans Language: English Journal: Braz. dent. j Journal subject: Dentistry Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: University of São Paulo/BR