An evaluation of p16INK4a expression in cervical intraepithelial neoplasia specimens, including women with HIV-1
Mem. Inst. Oswaldo Cruz
;
107(5): 571-577, Aug. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-643740
ABSTRACT
Although several studies have evaluated the role of p16INK4a as a diagnostic marker of cervical intraepithelial neoplasia (CIN) and its association with disease progression, studies regarding the role of p16INK4a in human immunodeficiency virus (HIV)-infected patients remain scarce. The present study was designed to determine the potential utility of p16INK4a as a diagnostic marker for CIN and invasive cervical cancer in HIV-positive and negative cervical specimens. An immunohistochemical analysis of p16INK4a was performed in 326 cervical tissue microarray specimens. Performance indicators were calculated and compared using receiving operating characteristics curve (ROC)/area under the curve. In HIV-1-negative women, the percentage of cells that was positive for p16INK4a expression was significantly correlated with the severity of CIN (p < 0.0001). A ROC curve with a cut-off value of 55.28% resulted in a sensitivity of 89%, a specificity of 81%, a positive predictive value of 91% and a negative predictive value of 78%. HIV-seropositive women exhibited decreased expression of p16INK4a in CIN2-3 specimens compared with HIV-negative specimens (p = 0.031). The ROC data underscore the potential utility of p16INK4a under defined conditions as a diagnostic marker for CIN 2-3 staging and invasive cervical cancer. HIV-1 infection, however, is associated with relatively reduced p16INK4a expression in CIN 2-3.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomarkers, Tumor
/
Uterine Cervical Dysplasia
/
Uterine Cervical Neoplasms
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Female
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2012
Type:
Article
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Fonte Medicina Diagnóstica/BR
/
Instituto Fernandes Figueira-Fiocruz/BR
/
Johns Hopkins Bloomberg School of Public Health/US
/
Ohio State University/US
/
Universidade Federal do Rio de Janeiro/BR
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