A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures
Mem. Inst. Oswaldo Cruz
;
107(5): 698-701, Aug. 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-643761
ABSTRACT
Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis. Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures, thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions.
Full text:
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Index:
LILACS (Americas)
Main subject:
Poliomyelitis
/
Poliovirus Vaccine, Oral
/
DNA Primers
/
Poliovirus
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2012
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fiocruz/BR
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