Characterizations of a new Cordyceps cicadae isolate and production of adenosine and cordycepin
Braz. j. microbiol
;
43(2): 449-455, Apr.-June 2012. ilus
Article
in English
| LILACS
| ID: lil-644458
ABSTRACT
Cordyceps is a fastidious pathogenic fungus infecting insects, and recent years have witnessed rapid progress in its medical properties. In this study, a wild isolate, C. cicadae MP12, was characterized through in vitro cultivation and its nuclear small-subunit (SSU) ribosomal DNA (rDNA) data. In vitro culture of C. cicadae MP12 was established by growing its fruiting bodies in a solid matrix. C. cicadae MP12 was inoculated into Cryptotympana atrata cicada pupae for in vivo culture, where the fungi developed its fruiting body as well. The contents of adenosine and cordycepin in dried fruiting bodies after culture were 1421.45µg/g and 1398.12 µg/g, respectively. Therefore, the established cultures from this study could be used for the production of various medically important metabolic substances.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
DNA, Ribosomal
/
Adenosine
/
Polymerase Chain Reaction
/
Cordyceps
/
Fungi
Limits:
Animals
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
China Agricultural University/CN
/
Zhejiang Agricultural and Forestry University/CN
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