Expression of phenazine biosynthetic genes during the arbuscular mycorrhizal symbiosis of Glomus intraradices
Braz. j. microbiol
;
43(2): 716-738, Apr.-June 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-644490
ABSTRACT
To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30% were homologous to fungal coding sequences, and 9% showed homology to previously characterized bacterial genes. Among them, GintPbr1a encodes a homolog to Phenazine Biosynthesis Regulator (Pbr) of Burkholderia cenocepacia, an pleiotropic regulatory protein that activates phenazine production through transcriptional activation of the protein D isochorismatase biosynthetic enzyme phzD (Ramos et al., 2010). Whereas GintPbr1a is expressed during the presymbiotic phase, the G. intraradices BE3 homolog of phzD (BGintphzD) is transcriptionally active at the time of the establishment of the arbuscular mycorrhizal symbiosis. DNA from isolated bacterial cultures found in spores of G. intraradices BE3 confirmed that both BGintPbr1a and BGintphzD are present in the genome of its potential endosymbionts. Taken together, our results indicate that spores of G. intraradices BE3 express bacterial phenazine biosynthetic genes at the onset of the fungal-plant symbiotic interaction.
Full text:
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Index:
LILACS (Americas)
Main subject:
Phenazines
/
Symbiosis
/
In Vitro Techniques
/
Base Sequence
/
Polymerase Chain Reaction
/
Reverse Transcriptase Polymerase Chain Reaction
/
Hyphae
/
Mycorrhizae
/
Enzymes
Type of study:
Prevalence study
/
Risk factors
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
Affiliation country:
Mexico
Institution/Affiliation country:
Centro de Investigación y Estudios Avanzados del IPN/MX
/
Centro de Investigación y Estudios Avanzados/MX
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