Callus induction and plant regeneration of Ulex europaeus
Electron. j. biotechnol
;
15(4): 7-7, July 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-646957
ABSTRACT
A callus induction and plant regeneration protocol was developed from leaf and thorn explants for the plant Ulex europaeus. Explants were incubated on 2 percent sucrose half-strength Murashige and Skoog Medium (MS) with various combinations of plant growth regulators and antioxidants. The best frequency of callus and shoot formation was obtained with 2,4-dichlorophenoxyacetic acid (2,4-D) 1 mg/l x kinetin (Kin) 0.2 mg/l (DK Medium; callus induction) and zeatin (Z) 1 mg/l (DK medium; shoot induction). Both media were supplemented with ascorbic acid 200 mg/l to prevent browning and death of the explants. The regenerated shoots transferred to rooting medium (half-strength MS Medium, 2 percent sucrose) showed rapid growth and development of roots (100 percent). Rooted plantlets were successfully transferred to soil in pots containing a 31 mixture of soil and vermiculite.
Full text:
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Index:
LILACS (Americas)
Main subject:
Regeneration
/
Ulex
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2012
Type:
Article
Affiliation country:
Chile
/
Germany
Institution/Affiliation country:
Max Planck Institute of Molecular Plant Physiology/DE
/
Universidad Técnica Federico Santa María/CL
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