Upregulation of matrix synthesis in chondrocyte-seeded agarose following sustained bi-axial cyclic loading
Clinics
;
67(8): 939-944, Aug. 2012. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-647799
ABSTRACT
OBJECTIVES:
The promotion of extracellular matrix synthesis by chondrocytes is a requisite part of an effective cartilage tissue engineering strategy. The aim of this in vitro study was to determine the effect of bi-axial cyclic mechanical loading on cell proliferation and the synthesis of glycosaminoglycans by chondrocytes in threedimensional cultures.METHOD:
A strain comprising 10% direct compression and 1% compressive shear was applied to bovine chondrocytes seeded in an agarose gel during two 12-hour conditioning periods separated by a 12-hour resting period.RESULTS:
The bi-axial-loaded chondrocytes demonstrated a significant increase in glycosaminoglycan synthesis compared with samples exposed to uni-axial or no loading over the same period (p<0.05). The use of a free-swelling recovery period prior to the loading regime resulted in additional glycosaminoglycan production and a significant increase in DNA content (p<0.05), indicating cell proliferation.CONCLUSIONS:
These results demonstrate that the use of a bi-axial loading regime results in increased matrix production compared with uni-axial loading.
Full text:
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Index:
LILACS (Americas)
Main subject:
Up-Regulation
/
Chondrocytes
/
Extracellular Matrix
/
Glycosaminoglycans
Limits:
Animals
Language:
English
Journal:
Clinics
Journal subject:
Medicine
Year:
2012
Type:
Article
/
Project document
Affiliation country:
Malaysia
Institution/Affiliation country:
University of Malaya/MY
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