Rapid in vitro plant regeneration from leaf explants of Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze
Biol. Res
;
45(2): 131-136, 2012. ilus, tab
Article
in English
| LILACS
| ID: lil-648571
ABSTRACT
An efficient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortified with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of high frequency microshoots in 30 days. The microshoots were successfully subcultured for shoot elongation and eventually for rooting on MS medium supplemented with indole-3-butyric acid (IBA) at 0.5mg/l. The regenerated plantlets were hardened under greenhouse conditions and transferred to garden, resulting in a 90% survival rate.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Plant Growth Regulators
/
Regeneration
/
Plant Shoots
/
Plant Leaves
/
Asteraceae
/
Organogenesis, Plant
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2012
Type:
Article
Affiliation country:
India
/
Israel
Institution/Affiliation country:
Karnatak University/IN
/
St. Joseph's College/IN
/
The Volcani Center/IL
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