Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
Int. braz. j. urol
; 38(4): 536-543, July-Aug. 2012. ilus, tab
Article
in En
| LILACS
| ID: lil-649448
Responsible library:
BR1.1
ABSTRACT
OBJECTIVE:
To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. MATERIALS ANDMETHODS:
After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry.RESULTS:
No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001).CONCLUSION:
The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.Key words
Full text:
1
Index:
LILACS
Main subject:
Biocompatible Materials
/
Urinary Bladder
/
Collagen
/
Vascular Endothelial Growth Factor A
/
Latex
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Int. braz. j. urol
Journal subject:
UROLOGIA
Year:
2012
Type:
Article