Glucokinase gene promoter -30G>A polymorphism: a cross-sectional association study with obesity, diabetes Mellitus, hyperlipidemia, hypertension and metabolic syndrome in an Iranian hospital / Polimorfismo -30G Oladi, Mohammad Reza; Behravan, Javad; Hassani, Mitra; Kassaeian, Jamal; Sahebkar, Amirhossein; Tavallaie, Shima; Paydar, Roghayeh; Saber, Hamidreza; Esmaeili, Habib Allah; Azimi-Nezhad, Mohsen; Ferns, Gordon; Ghayour-Mobarhan, Majid. Oladi, Mohammad Reza; Mashhad University of Medical Sciences. Cardiovascular Research Center. Mashhad. IR Behravan, Javad; Mashhad University of Medical Sciences. Biotechnology Research Center. Mashhad. IR Hassani, Mitra; Mashhad University of Medical Sciences. Biotechnology Research Center. Mashhad. IR Kassaeian, Jamal; Mashhad University of Medical Sciences. Biotechnology Research Center. Mashhad. IR Sahebkar, Amirhossein; Mashhad University of Medical Sciences. Cardiovascular Research Center. Mashhad. IR Tavallaie, Shima; Mashhad University of Medical Sciences. Biotechnology Research Center. Faculty of Medicine. Mashhad. IR Paydar, Roghayeh; Mashhad University of Medical Sciences. Biotechnology Research Center. Faculty of Medicine. Mashhad. IR Saber, Hamidreza; Mashhad University of Medical Sciences. Biotechnology Research Center. Faculty of Medicine. Mashhad. IR Esmaeili, Habib Allah; Mashhad University of Medical Sciences. Faculty of Medicine. Departament of Community Medicine and Public Health. Mashhad. IR Azimi-Nezhad, Mohsen; Mashhad University of Medical Sciences. Cardiovascular Research Center. Mashhad. IR Ferns, Gordon; University of Keele. Institute for Science and Technology in Medicine. Keele. GB Ghayour-Mobarhan, Majid; Mashhad University of Medical Sciences. Biotechnology Research Center. Faculty of Medicine. Mashhad. IR Rev. nutr ; 25(4): 487-495, jul.-ago. 2012. tab Article in English | LILACS | ID: lil-650722

ABSTRACT

OBJECTIVE: A -30G>A single nucleotide polymorphism in the promoter region of the glucokinase gene has been previously associated with obesity, insulin resistance and diabetes. The present study aimed to evaluate the association of this polymorphism with obesity and its comorbidities in a population from Northeast Iran. METHODS: Five hundred and forty-two subjects aged 18 to 65 years were included in the study and divided into normal (BMI<25, n=220), overweight (2530, n=187) groups. All subjects were genotyped for the -30G>A polymorphism using the polymerase chain reaction-restriction fragment length polymorphism technique.

RESULTS:

The genotypes and allele frequencies of the three groups did not differ significantly (p>0.05). When the study population was categorized according to diabetes mellitus, hyperlipidemia, hypertension and metabolic syndrome status, no significant difference in -30G>A genotypes and alleles was found between the subgroups with and without these disorders (p>0.05), apart from a significantly higher frequency of the G allele in the hyperlipidemic vs. non-hyperlipidemic subgroup (p<0.05).

CONCLUSION:

The findings of the present study do not support an association between the -30G>A polymorphism and high body mass index in the Iranian population.

RESUMO

OBJETIVO: O polimorfismo de nucleotídeo único -30G>A, na região promotora do gene da glucoquinase, já foi associado à obesidade, resistência insulínica e diabete. O objetivo deste estudo foi avaliar a associação deste polimorfismo com a obesidade e suas comorbidades em uma população do nordeste iraniano. MÉTODOS: Quinhentos e quarenta e dois indivíduos com idades entre 18 e 65 anos foram divididos em três grupos normal (BMI<25, n=220), sobrepeso (2530, n=187). Todos os indivíduos foram genotipados para o polimorfismo -30G>A através da técnica da reação em cadeia da polimerase - polimorfismo do comprimento do fragmento de restrição.

RESULTADOS:

As frequências dos genótipos e alelos dos 3 grupos não diferiram entre si (p>0,05). Quando a população de estudo foi categorizada de acordo com a presença de diabete, hiperlipidemia, hipertensão arterial e síndrome metabólica, os genótipos e alelos -30G>A dos subgrupos com e sem essas doenças não diferiram entre si (p>0,05), exceto por uma frequência maior do alelo G no grupo de hiperlipidêmicos quando comparados aos não hiperlipidêmicos (p<0,05).

CONCLUSÃO:

Os achados do presente estudo não confirmam uma associação entre o polimorfismo -30G>A e excesso de peso na população iraniana.