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Evaluation of rapid tests for human immunodeficiency virus as a tool to detect recent seroconversion
Girardi, Samira Barboza; Barreto, Angela Maria Egydio de Carvalho; Barreto, Claudia Cortese; Proietti, Anna Barbara; Carvalho, Silvia Maia Farias de; Loureiro, Paula; Sabino, Ester Cerdeira.
  • Girardi, Samira Barboza; Universidade Federal de São Paulo. Escola Paulista de Medicina. São Paulo. BR
  • Barreto, Angela Maria Egydio de Carvalho; Fundação Pró-Sangue Hemocentro de São Paulo. São Paulo. BR
  • Barreto, Claudia Cortese; Fundação Pró-Sangue Hemocentro de São Paulo. São Paulo. BR
  • Proietti, Anna Barbara; Fundação Centro de Hematologia e Hemoterapia de Minas Gerais. Belo Horizonte. BR
  • Carvalho, Silvia Maia Farias de; Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti. Rio de Janeiro. BR
  • Loureiro, Paula; Fundação Hemope. Recife. BR
  • Sabino, Ester Cerdeira; Universidade de São Paulo. Institute of Tropical Medicine. Department of Infectious Disease. São Paulo. BR
Braz. j. infect. dis ; 16(5): 452-456, Sept.-Oct. 2012. tab
Article in English | LILACS | ID: lil-653434
ABSTRACT
The identification of recent HIV infection is important for epidemiological studies and to monitor the epidemic. The objective of this study was to evaluate two rapid tests that are easily available to the Brazilian scientific community for using as markers of recent HIV infection. The Rapid Test - HIV-1/2 Bio-Manguinhos (Bio-Manguinhos/Fiocruz, Brazil) and the Rapid Check HIV 1&2 (NDI-UFES, Center for Infectious Diseases, Universidade Federal do Espírito Santo) were tested, using 489 samples with HIV positive serology, from blood donors, previously classified as recent or long-term infection by serological testing algorithm for recent HIV seroconversion (STARHS) or LS-HIV Vitros assay methods. The samples were diluted prior to testing (150 and 1100 for the Rapid Test - HIV-1/2 Bio-Manguinhos, and 1500 and 1600 for the Rapid Check HIV 1&2). Negative samples were considered recent infection, whereas those showing any color intensity were associated with long-term infection. The best dilutions were 1100 for HIV-1/2 Bio-Manguinhos test (Kappa = 0.840; overall agreement = 0.93), and 1500 for the Rapid Check HIV 1&2 (Kappa = 0.867; overall agreement = 0.94). The results suggest that both rapid tests can be used to detect recent seroconversion.
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Full text: Available Index: LILACS (Americas) Main subject: Reagent Kits, Diagnostic / HIV Antibodies / HIV Infections / HIV-1 / HIV-2 Type of study: Diagnostic study / Evaluation studies / Prognostic study Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Centro de Hematologia e Hemoterapia de Minas Gerais/BR / Fundação Hemope/BR / Fundação Pró-Sangue Hemocentro de São Paulo/BR / Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti/BR / Universidade Federal de São Paulo/BR / Universidade de São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Reagent Kits, Diagnostic / HIV Antibodies / HIV Infections / HIV-1 / HIV-2 Type of study: Diagnostic study / Evaluation studies / Prognostic study Limits: Humans Country/Region as subject: South America / Brazil Language: English Journal: Braz. j. infect. dis Journal subject: Communicable Diseases Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: Fundação Centro de Hematologia e Hemoterapia de Minas Gerais/BR / Fundação Hemope/BR / Fundação Pró-Sangue Hemocentro de São Paulo/BR / Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti/BR / Universidade Federal de São Paulo/BR / Universidade de São Paulo/BR