Cytotoxic response of two cell lines exposed in vitro to four endodontic sealers
Braz. j. oral sci
;
11(2): 135-140, abr.-jun. 2012. ilus
Article
in English
| LILACS, BBO
| ID: lil-654835
ABSTRACT
Aim:
To investigate the cytotoxicity of four endodontic sealers with different bases Epiphany(EPH), AH Plus (AHP), Sealer 26 (S26) and Endofill (ENF) on human foreskin fibroblasts(HFF) and mouse macrophages (J774/G8).Methods:
Cells were placed in direct contact withfreshly prepared endodontic sealers in polypropylene tubes. The cells were incubated for 24, 48and 72 h. Cytotoxicity was assessed using the MTT assay (cell viability) and Griess reagent (NOrelease).Results:
On the HFF cultures, EPH showed the lowest viability levels of all four sealersat 24 h (p<0.05), but over time (72h), EPH lessened its toxic levels in a similar pattern as the otherthree materials (p>0.05). The viability of all four sealers on the macrophage cultures showed nostatistically significant difference over time, except between EPH and AHP at 72 h (p<0.05).Although uniformity was not detected in macrophage and fibroblast release of NO in response tosealers over time, a trend of increased NO levels for EPH (p<0.05) was observed.Conclusions:
The response pattern varied depending on time and type of cell line used for analysis, althoughthe results indicate a higher cytotoxicity for EPH in short-term tests.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Fibroblasts
/
Macrophages
Language:
English
Journal:
Braz. j. oral sci
Journal subject:
Dentistry
Year:
2012
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Triângulo Mineiro/BR
/
Federal University of Uberlândia/BR
/
University of Campinas/BR
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