Your browser doesn't support javascript.
loading
Comparison of five methods for oxacillin susceptibility testing of Staphylococcus aureus isolates from cystic fibrosis patients / Comparação entre cinco métodos para avaliação de susceptibilidade à oxacilina em cepas de Staphylococcus aureus isoladas de pacientes com fibrose cística
Mimica, Marcelo J.; Carvalho, Rozane L.; Berezin, Eitan N.; Damaceno, Neiva; Caiaffa-Filho, Hélio H..
  • Mimica, Marcelo J.; Santa Casa School of Medicine. Department of Pathology. Division of Microbiology. São Paulo. BR
  • Carvalho, Rozane L.; Santa Casa School of Medicine. Department of Pathology. Division of Microbiology. São Paulo. BR
  • Berezin, Eitan N.; Santa Casa School of Medicine. Department of Pediatrics. Division of Infectious Diseases. São Paulo. BR
  • Damaceno, Neiva; Santa Casa School of Medicine. Department of Pediatrics. Division of Pulmonology. São Paulo. BR
  • Caiaffa-Filho, Hélio H.; FMUSP. Hospital das Clinicas. Laboratory of Medical Investigation. São Paulo. BR
Rev. Inst. Med. Trop. Säo Paulo ; 54(6): 305-306, Nov.-Dec. 2012.
Article in English | LILACS | ID: lil-656263
ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) are now a worldwide problem. Cystic fibrosis (CF) patients are commonly colonized and infected by MRSA. Accurate oxacillin susceptibility testing is mandatory for the adequate management of these patients. We performed a comparison of the accuracy of different tests in CF isolates, including methicillin-susceptible S. aureus and MRSA with different SCCmec types, and using the mecA gene as the gold-standard. The sensitivity and specificity of oxacillin disc, Etest, and oxacillin agar screening plate were 100%. Sensitivity of the cefoxitin disc was 85% and specificity was 100%. For clinically relevant isolates, laboratories may consider the use of a combination of two phenotypic methods.
RESUMO
Staphylococcus aureus resistentes à oxacilina (MRSA) são, atualmente, um problema global. Pacientes com fibrose cística (FC) são frequentemente colonizados e infectados por MRSA. A realização de testes de susceptibilidade acurados é extremamente importante para o manejo da terapia antimicrobiana nesses indivíduos. Nesse estudo, realizamos comparação entre as acurácias de diversos testes de susceptibilidade à oxacilina, em cepas de S. aureus isoladas de pacientes com fibrose cística, tanto sensíveis como resistentes à oxacilina, com diferentes tipos de SCCmec, e utilizando a detecção do gene mecA como método padrão. A sensibilidade e a especificidade do disco de oxacilina, do Etest, e da placa de agar screening com oxacilina foram de 100%. A sensibilidade do disco de cefoxitina foi 85%, com especificidade de 100%. Em cepas clinicamente relevantes, a utilização combinada de mais de um método deveria ser considerada.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oxacillin / Staphylococcal Infections / Staphylococcus aureus / Bacterial Proteins / Cystic Fibrosis / Anti-Bacterial Agents Type of study: Diagnostic study Limits: Humans Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: FMUSP/BR / Santa Casa School of Medicine/BR

Similar

MEDLINE

...
LILACS

LIS


Full text: Available Index: LILACS (Americas) Main subject: Oxacillin / Staphylococcal Infections / Staphylococcus aureus / Bacterial Proteins / Cystic Fibrosis / Anti-Bacterial Agents Type of study: Diagnostic study Limits: Humans Language: English Journal: Rev. Inst. Med. Trop. Säo Paulo Journal subject: Tropical Medicine Year: 2012 Type: Article Affiliation country: Brazil Institution/Affiliation country: FMUSP/BR / Santa Casa School of Medicine/BR