Nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis

Macedo, Maíra Bidart; Groll, Andrea Von; Fissette, Krista; Palomino, Juan Carlos; Silva, Pedro Eduardo Almeida da; Martin, Anandi

Macedo, Maíra Bidart; Groll, Andrea Von; Fissette, Krista; Palomino, Juan Carlos; Silva, Pedro Eduardo Almeida da; Martin, Anandi.

Macedo, Maíra Bidart; Universidade Federal do Rio Grande. Faculdade de Medicina. Laboratório de Micobactérias. Rio Grande. BR
Groll, Andrea Von; Universidade Federal do Rio Grande. Faculdade de Medicina. Laboratório de Micobactérias. Rio Grande. BR
Fissette, Krista; Institute of Tropical Medicine. Antwerp. BE
Palomino, Juan Carlos; Institute of Tropical Medicine. Antwerp. BE
Silva, Pedro Eduardo Almeida da; Universidade Federal do Rio Grande. Faculdade de Medicina. Laboratório de Micobactérias. Rio Grande. BR
Martin, Anandi; Institute of Tropical Medicine. Antwerp. BE

Braz. j. microbiol ; 43(3): 981-983, July-Sept. 2012. tab

Article in English | LILACS | ID: lil-656662

ABSTRACT

ABSTRACT

We validated the nitrate reductase assay (NRA) for the detection of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) using sodium nitrate (NaNO3) in replacement of potassium nitrate (KNO3) as nitrate source. NaNO3 is cheaper than KNO3 and has no restriction on use which facilitates the implementation of NRA to detect MDR-TB.

Subject(s)

Humans; Kali Nitricum/analysis; Kali Nitricum/isolation & purification; Mycobacterium Infections; Mycobacterium/isolation & purification; Nitrate Reductases/analysis; Nitrate Reductases/isolation & purification; Tuberculosis, Multidrug-Resistant; Biological Assay; Immunity, Innate; Methods

Multidrug resistant, sodium nitrate; Nitrate reductase assay; Tuberculosis

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Full text: Available Index: LILACS (Americas) Main subject: Kali Nitricum / Tuberculosis, Multidrug-Resistant / Mycobacterium / Mycobacterium Infections / Nitrate Reductases Type of study: Diagnostic study Limits: Humans Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2012 Type: Article Affiliation country: Belgium / Brazil Institution/Affiliation country: Institute of Tropical Medicine/BE / Universidade Federal do Rio Grande/BR

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