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Isolation and characterization of canine parvovirus type 2c (CPV-2c) from symptomatic puppies
Puentes, R; Eliopulos, N; Pérez, R; Franco, G; Sosa, K; Bianchi, P; Furtado, A; Hübner, S. O; Esteves, P. A.
  • Puentes, R; UdelaR. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo. UY
  • Eliopulos, N; UdelaR. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo. UY
  • Pérez, R; UdelaR. Facultad de Ciencias. Sección Genética evolutiva. Montevideo. UY
  • Franco, G; UdelaR. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo. UY
  • Sosa, K; UdelaR. Facultad de Ciencias. Sección Genética evolutiva. Montevideo. UY
  • Bianchi, P; UdelaR. Facultad de Ciencias. Sección Genética evolutiva. Montevideo. UY
  • Furtado, A; UdelaR. Facultad de Veterinaria. Departamento de Ciencias Microbiológicas. Montevideo. UY
  • Hübner, S. O; Universidade Federal de Pelotas. Faculdade de Veterinaria. Pelotas. BR
  • Esteves, P. A; Empresa Brasileira de Pesquisa Agropecuária. Concórdia. BR
Braz. j. microbiol ; 43(3): 1005-1009, July-Sept. 2012. ilus
Article in English | LILACS | ID: lil-656665
ABSTRACT
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs.
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Full text: Available Index: LILACS (Americas) Main subject: Phylogeny / In Vitro Techniques / Base Sequence / Genome, Viral / Parvovirus, Canine / Parvoviridae Infections / Diarrhea Type of study: Diagnostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2012 Type: Article Affiliation country: Brazil / Uruguay Institution/Affiliation country: Empresa Brasileira de Pesquisa Agropecuária/BR / UdelaR/UY / Universidade Federal de Pelotas/BR

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Full text: Available Index: LILACS (Americas) Main subject: Phylogeny / In Vitro Techniques / Base Sequence / Genome, Viral / Parvovirus, Canine / Parvoviridae Infections / Diarrhea Type of study: Diagnostic study Limits: Animals Language: English Journal: Braz. j. microbiol Journal subject: Microbiology Year: 2012 Type: Article Affiliation country: Brazil / Uruguay Institution/Affiliation country: Empresa Brasileira de Pesquisa Agropecuária/BR / UdelaR/UY / Universidade Federal de Pelotas/BR