Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
Braz. j. microbiol
;
43(3): 1128-1136, July-Sept. 2012. tab
Article
in English
| LILACS
| ID: lil-656683
ABSTRACT
A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacteriophages
/
Culture Media
/
Milk
/
Microbial Viability
/
Listeria monocytogenes
Type of study:
Diagnostic study
/
Practice guideline
Limits:
Humans
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2012
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Instituto Oswaldo Cruz/BR
/
Universidade Estadual da Bahia/BR
/
Universidade Federal da Bahia/BR
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